A specific and rapid method for detecting Bacillus and Acinetobacter species in Daqu

Daqu is a spontaneous, solid-state cereal fermentation product used for saccharification and as starter culture Chinese Baijiu production. Bacillus Acinetobacter , two dominant microbial genera in Daqu, produce enzymes organic acids that influence the quality. However, there are no rapid analytical methods detecting . We designed primers specific to perform genetic comparisons using 16 S rRNA. After amplification of polymerase chain reaction primers, high-throughput sequencing was performed detect strains The results showed effective rates were 86.92% 79.75%, respectively. Thus, we have devised assessed method accurately identify species associated with which can also hold significance bacterial typing identification.